Hpa I

Mpa I

Digestion of the restriction enzyme with a reliable restriction endonuclease, HpaI. The Hpa I is a restriction enzyme purified by an E. coli strain carrying the cloned Hpa I gene of Haemophilus parainfluenzae.


Following reactants are included with this product: 1 microg ? The amount of enzym needed to ingest 1 microg of genomic protein in 1 hr at 37°C and a 50 microlitre overall response time. In the case of non thermally inactivatable genes, we suggest the use of a purification pillar (e.g. Monarch PCR & PCR kit ), carrying out the reagent on an aggressive agent and subsequent isolation of the genes (we suggest Monarch Gels Isolation Kit) or phenol/chloroform isolation.

The stellar action can be caused by prolonged intestinal transit, a high enzymatic content or a glycerine content of > 5%. Below is a listing of the Material Safety Data Sheets (SDS) that are applicable to this material safety device. Only for research use. It is not designed for human or animal use.

Haemophilus Hpa I from Haemophilus paraainfluenza

In the PCR mixture (Taq Proteinase Buffer) the specificity is 100%. PCR mixture included primer, 10 mM Tris-HCl (pH 8.3, 20 C), 50 mM MgCl, 1.5 mM KCl2, 200 ?M PCR mixture containing 2.5Haq, 2.5U Daq protein copolymer. The Hpa I detects the scene GTT?*A°AC and creates segments with dull ends.

For 16 hrs, SuRE/Cut Buffer A is incorporated in 50?g ?l The number of Enzymatic Modules that do not alter the enzyme-specific sample is indicated in the Analytical Report. Missing activity of aliquots Approximately 5?g[3H] marked calbsthymus DNNA are incubated for 4 h at +37°C for 4 h at a combined capacity of 110?l 50 mM Tris-HCl, 10 mM and 1mM dithioerythritol, pH about 7.5.

A mechanism is the enzymatic action that 1 ?g ?l splits 1 fully in one hr at +37 ºC in a whole of 25 ?l (1x) SuRE/Cut Puffer A. The enzymes produces ends that are mutually interchangeable with each dull end. Enzymes are not known to contain isoshizomers. Ligations and reconstitution assaysHpa I samples obtained by full indigestion from 1 ?g ?l are combined with 1 T4 DNA ligase in a 10 ?l vol. by incubating for 16 h at +4°C in 66 mM Tris-HCl, 5 mM and 5 mM ATP, pH 7.5 (at +20°C), resulting in >80% recycling of 1 x Hpa I samples.

Re-trimming with Hpa I results in >95% of the characteristic sample of Hpa I segments.

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